Phospho- ser/thr pka substrate antibody

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August undefined, 2024

WebSep 1, 2005 · Phospho- (Ser/Thr) PKA substrate antibody was from Cell Signaling Technology (Catalog number 9621). Speract was from Peninsula Laboratories. Anti-bindin antibody was prepared as described ( Moy and Vacquier, 1979 ). All other reagents and chemicals were from Sigma. WebPhospho-(Ser/ Thr) PKA antibody substrates include Apaf-1, CAMKII, Caspase-10, Caspase-2, CREB, and NMDAR1, among others (see Cell Signaling #9621). Alpha--tubulin antibody was purchased from ...

Phospho-(Ser/Thr) PKA Substrate Antibody Cell Signaling …

WebPhospho- (Ser/Thr) PKA Substrate Antibody. *On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca. WebThe inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. normale temperatur baby

Phospho-PKA alpha (Ser338) Polyclonal Antibody - Thermo Fisher Scie…

WebProtein kinase A (PKA), also known as cAMP-dependent protein kinase (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of … WebPartially purified Xenopus EF-2 (xEF-2) was phosphorylated by PKA in vitro and this phosphorylation was detected by Western blotting using PKA substrate antibody. The phosphorylation of Thr-57 in xEF-2 (corresponding to Thr-56 of the mammalian enzyme) was detected in the partially purified xEF-2 from the resting oocytes, but this xEF-2 did not ... WebPhospho-(Ser/Thr) PKA Substrate Antibody 可检测包含磷酸-丝氨酸/苏氨酸残基（在 -3 位点有精氨酸）的肽和蛋白。它是一种有用的工具，可用来识别 AGC 家族激酶的底物，包括 … how to remove people from cashapp

NeuroMab™ Anti-Phospho-PKA C (Thr197) Antibody, Clone N3292

Phospho-PKA alpha/beta (Thr197) Recombinant Polyclonal Antibody …

WebPhospho-PKA substrate antibody AD0182 (supplied by Cell Signaling Technology) Phospho-PKA substrate antibody is an affinity-purified rabbit polyclonal antibody. It is highly specific for phosphorylated threonine with arginine at the –3 position. It also recognizes phospho-serine with arginine at the –2 and –3 position. WebA good control is the effect of the H89 inhibitor (as well as a known agonist such as dibutyryl cAMP). A possible PKA substrates is CREB (Ser 133) for which there are good phospho-specific antibodies. normale schuhe mit rollenWebPhospho-(Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine residue with arginine at the -3 position. It is a useful tool in identifying substrates of AGC family kinases, … normale witze

"WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … " - Phospho- ser/thr pka substrate antibody

Phospho- ser/thr pka substrate antibody

Phospho-PKA alpha (Ser338) Polyclonal Antibody - Thermo Fisher Scie…

WebBuy pka antibodies from Santa Cruz Biotechnology, Inc. Monoclonal antibodies are available to most protein immunogens. ... PKA Phosphopeptide Substrate Antibody (15B12) sc-65491: mouse IgG 1: N/A: ELISA: NSR: NEW (1) ... Phospho-Specific PKA Antibodies PRODUCT NAME CATALOG # ISOTYPE EPITOPE APPLICATIONS Species Citations WebIn order to study the phosphorylated proteins in the resting Xenopus laevis oocytes, the proteins detected by Western blotting using phospho-(Ser/Thr) PKA substrate antibody …

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WebPhospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif. The antibody also … WebIn addition, a phospho- (Ser/Thr) PKA substrate antibody detected bands on immunoblot analysis of trans-sialidase antibody precipitated proteins from parasite lysate and the …

WebPhospho-PKA alpha (Ser338) Polyclonal Antibody detects endogenous levels of PKA alpha only when phosphorylated at Ser338. Target Information Protein kinase A (PKA, cAMP … WebThis antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position. Visit thermofisher.com to view references and figures.

WebAnti-Phospho - (Ser/Thr) antibody Description Rabbit polyclonal to Phospho - (Ser/Thr) Host species Rabbit Tested applications Suitable for: ELISA, Dot blot more details Species … WebSee all Phospho - (Ser/Thr) Phe primary antibodies Description Rabbit polyclonal to Phospho - (Ser/Thr) Phe Host species Rabbit Tested applications Suitable for: ELISA, IP, WB, ICC/IF …

WebJun 25, 2015 · A phospho-(Ser/Thr) PKA substrate antibody (Cell Signaling Technology) was used to detect in vivo phosphorylation of CP. Constructions of plasmids.

WebImmunodetection of phospho- (Ser/Thr) PKA substrates shows that phosphorylated proteins are localized differently in spermatozoa of carp and sterlet. Strong phosphorylation of PKC substrate was observed in flagella of sterlet spermatozoa, whereas in carp sperm, PKC substrates were lightly phosphorylated in the midpiece and flagella. normal erupted wizdom teethWebDec 15, 2009 · (C) Thr-90-phosphorylation status of Hsp90α in the CM and TCL. pT90-Hsp90α was detected using anti-phospho-(Ser/Thr) PKA substrate antibody. (D) Secretion and Thr-90-phosphorylation status of Hsp90α upon overexpression of Flag-tagged PKA (Left) or upon siRNA treatment (Right) detected by Western blotting and ELISA. PKA was … normal evaporation for poolWebPhosphoserine/threonine/tyrosine Antibody (61-8300) in ELISA. Direct ELISA analysis of Phosphotyrosine, Phosphoserine, and Phosphothreonine was performed by coating wells of a 96-well plate with 100 µL per well of … how to remove people from microsoft familyWebOct 19, 2005 · Four hundred microliters NP40-solubilized sperm lysate (2 mg/ml) was incubated with 4 μl of the Phospho-(Ser/Thr) PKA substrate antibody and 40 μl of 50% protein A-Sepharose CL4B beads. Precipitated immunocomplexes, bound to the protein A beads, were washed four times in NP40 lysis buffer without phosphatase inhibitors and … how to remove people from fb messengerWebInactivation of a Gαs–PKA tumour suppressor pathway in skin stem cells initiates basal-cell carcinogenesis normal evd waveformWebThe NeuroMab™ anti-Phospho-PKA C (Thr197) antibody detects endogenous levels of PKA C (-α, -β, and -γ) only when phosphorylated at Thr197. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr197 of human PKA C protein. Store at -20°C. Do not aliquot the antibody. normale temperatuur hondWebPolyclonal Antibody for studying PKA (RRXt) substrate motif phosphate. Cited in 210 publications. Validated for WB, IP, IHC, E. Available in 2 sizes. Highly specific and … normal esophageal constrictions