Ezh2 antibody
TīmeklisMonoclonal antibody is produced by immunizing animals with Ezh2 recombinant protein containing human Ezh2 residues 351-453. Background The polycomb group (PcG) … Tīmeklis2024. gada 23. jūl. · Dual luciferase reporters were used to test the regulation between PDK1 and miR-148a. HDAC2 and EZH2 were found to regulate miR-148a expression through Western blotting assays, qRT-PCR and co-immunoprecipitation assays. ... 1% BSA/PBST containing antibodies against Vimentin (1:100) and E-cadherin (1:100) …
Ezh2 antibody
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TīmeklisChIP results obtained with the Diagenode monoclonal antibody directed against EZH2. ChIP assays were performed using 2.5 μg of the Diagenode monoclonal antibody against EZH2 (Cat. No. MAb-180-050) on sheared chromatin from 5 million E14 mouse embryonic stem and from SUZ12-/- cells, used as a negative control. IgG was used … Tīmeklis2024. gada 24. febr. · ENX-1 Antibody (D-8) is a mouse monoclonal IgG 2b κ ENX-1 antibody, cited in 6 publications, provided at 200 µg/ml. specific for an epitope mapping between amino acids 63-102 within an internal region of ENX-1 of human origin. ENX-1 Antibody (D-8) is recommended for detection of ENX-1 of mouse, rat and human …
TīmeklisS. M. Actin is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative. Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product. TīmeklisDescription. Anti-EZH2 Antibody. Alternate Names. KMT6. Enx1. Enhancer of Zeste Homologue 2. Background Information. Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the …
TīmeklisEZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential … TīmeklisSodium azide is highly toxic. EZH2 phospho Thr345 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of EZH2 phospho Thr345 antibody. Modified and unmodified peptide were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. Decreasing amounts of peptide were spotted in each row.
TīmeklisImmunocytochemistry analysis of Phospho-EZH2 (Thr345) in A549 cells (heat shock treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody ( Product # PA5-114574 ). Secondary staining was applied with mouse anti-beta …
TīmeklisFigure 3 Intermittent hyperglycemia causes EZH2 coupling with PRC2 proteins, leading to H3K27 trimethylation at promoter regions of the KLF2 and KLF4 genes. ( A ) Co … lowest price dell xps 15TīmeklisPhosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing. Sumoylated. Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex. janet thompson obituary 2021lowest priced electric blanketsTīmeklisSynthetic peptide within Human KMT6/ EZH2 aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If … lowest price dell business laptopTīmeklisEZH2 Antibody (MA5-15101) in WB. Knockdown of EZH2 was achieved by transfecting Hep G2 cells with EZH2 specific siRNAs (Silencer® select Product # s4918, s4917). … janet thompson obituaryTīmeklisThe antibodies are designated mAB for monoclonal and pAb for polyclonal. The release of the Human Protein Atlas in which the antibody was first published. All assays … lowest price delivered monitorTīmeklisThese antibodies have been verified by Knockdown, Relative expression and Cell treatment to confirm specificity to EZH2. Find the EZH2 antibody that fits your … janet thompson obituary michigan