Cy5 extinction coefficient chart

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August undefined, 2024

WebExtinction coefficient: 265,000 cm-1M-1 Spectrally similar dyes: Cy5 Molecular weight: ∼1250 Typical Conjugation Reaction The protein should be dissolved at a concentration of 50-100 µM in a suitable buffer (10-100 mM phosphate, Tris, or HEPES) at pH 7.0-7.5. In this pH range, the protein thiol groups are sufficiently nucleophilic that they ... WebApr 28, 2009 · Cy5.5 is a NIR fluorescence dye with an absorbance maximum at 675 nm and an emission maximum at 694 nm with a high extinction coefficient of 250,000 M-1 cm-1. Synthesis [ PubMed ]

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WebThe molar extinction coefficient (ε) for Cy5.5 (Cyanine-5.5) is 250000 cm-1M-1 . To request additional information and datasets for Cy5.5 (Cyanine-5.5), please contact [email protected]. Unless otherwise specified, molar extinction coefficient (ε) values are typically measured in ddH 2 O or PBS. WebFL3 Red PE-Cy5a PerCP PerCP-Cy5.5 PE-Cy7 Red Diode 635 FL4 Red APCa Alexa Fluor® 647 *BD FACSCanto™ flow cytometry system Solid State 488 Green FITC Alexa … lowes quickset

Extinction Coefficient [Cy5.5 (Cyanine-5.5)] AAT Bioquest

WebInitial brightness indicates the relative brightness of the fluorophore, which is a product of the molar extinction coefficient and quantum yield, both of which are specific to each fluor. Fluorophores with high initial brightness can be used to detect lower-abundance targets WebIt results in the loss of signal for PerCP but not for its acceptor, Cy5.5 whose fluorescence can be detected in the Alexa Fluor® 700 channel. Figure 2. A) Fluorescence intensity of PerCP/Cy5.5 anti-CD14 antibody staining … Websolution and molar extinction coefficient, please see the related Tech Tip #6: Extinction coefficients. • Each kind of fluorescent dye molecule absorbs maximally at a particular … lowes queen bed frame bookcase

4.2: Cyanine Dyes - Chemistry LibreTexts

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WebFluorophore Brightness Index Fluorophore Ex (nm) Em (nm) Filter Brightness Brilliant Violet 421™ 405 421 450/50 5 PE 496, 565 575 585/40 5 APC 650 660 660/20 5 PE/Cy5 496, 565 670 660/20 5 Brilliant Violet 605™ 405 603 610/20 4 Alexa Fluor® 647 650 668 660/20 4 PE/Cy7 496, 565 774 780/60 4 Brilliant Violet 711™ 405 711 710/50 3 Brilliant … WebCy5.5. Cyanine 5.5 (Cy5.5) is a fluorescent dye that belongs to the Cyanine family of synthetic polymethine dyes. ... Extinction coefficient (260nm) 28.8 mM-1 cm-1: Cy5.5 as an internal Cy5-dT; MW: 1059 g/mol: Excitation maximum: 683 nm: Emission maximum: 705 nm: Extinction coefficient (260nm) james walling obituaryWebApr 21, 2024 · Figure 4.2.2: Absorption spectra of 3 cyanine dyes constructed from data in the paper by W.T. Simpson, J. Chem. Phys. Series A, 1948, 16, p 1124. R = CH3 for each dye. Dye I has 3 carbon atoms and the absorption maximum is at 309 nm, dye II has 5 carbon atoms and the absorption maximum, λmax, is at 409 nm, and dye III has 7 … lowes queensland

"WebJan 30, 2024 · The constant \(\epsilon\) is called molar absorptivity or molar extinction coefficient and is a measure of the probability of the electronic transition. On most of the diagrams you will come across, the absorbance ranges from 0 to 1, but it can go higher than that. An absorbance of 0 at some wavelength means that no light of that particular ... " - Cy5 extinction coefficient chart

Cy5 extinction coefficient chart

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Web20 rows · Alexa Fluor dyes—Emission spectra. Figure 1. Emission spectra for Alexa Fluor dyes. Table 1. Properties of Alexa Fluor Dyes. * Typical emission color seen through the … Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation … Alexa Fluor 488™ phalloidin is a high-affinity filamentous actin (F-actin) probe … Extinction coefficient: 73,000 cm-1M-1 Spectrally similar dyes: FITC, Oregon … • Far-red-fluorescent Alexa Fluor 647—excitation and emission maxima of … • Far-red-fluorescent Alexa Fluor 647—excitation and emission maxima of … Extinction coefficient: 72,000 cm-1M-1 Spectrally similar dyes: Fluorescein … Alexa Fluor Plus 488 allows you to see greater detail compared to traditional … WebCyanine 5 (Cy5) can be efficiently used in multiplex PCR in combinations. Cy5 is a fluorescent dye that belongs to the Cyanine family of synthetic polymethine dyes and it represents an excellent alternative to WellRED dye D4 and LIZ. Metabion offers the Cy5 modification in its DNA and RNA portfolio. Click here « back to overview

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WebExtinction Coefficient 250,000 Flow Cytometry Laser Line 633 or 635 nm Microscopy Laser Line 633 or 635 nm Spectrally Similar Dyes Alexa Fluor® 647, CF™ 647 Dye, DyLight™649 Molecular weight 787.96 (protonated) … Web21 rows · Extinction Coefficient ‡ Alexa Fluor 350: 346: 442: Blue: 19,000: Alexa Fluor …

WebThe right panel shows Cy5-labeled antibodies. Antibodies were labeled with various degree of labeling (DOL), i.e., Cy5-to-antibody ratio. The DOL of antibody–dye conjugates was … WebCy5 650 670 red Alexa fluor 660 663 690 red Cy5.5 675 694 red TruRed 490;675 695 red Alexa fluor 680 679 702 red Cy7 743 770 red Nucleic acid probes: Dye Absorbance Wavelength Emission Wavelength Visible color DAPI 345 455 blue Hoechst 33258 345 478 blue SYTOX blue 431 480 blue Hoechst 33342 343 483 blue YOYO-1 509 509 green …

WebJul 1, 2006 · This expression is identical to the “Lambert–Beer law” which is used to describe the radiation attenuation in homogeneous solutions which can written as (2) I = I 0 1 0 - ε xc, where ε is called the molar extinction coefficient. Comparing Eqs. (1), (2), we have ε = σ N A Log 10 e = 0.4343 σ N A = 0.4343 M μ ρ, where M is the molar ... WebThe molar extinction coefficient (ε) for Cy5.5 (Cyanine-5.5) is 250000 cm-1M-1 . Unless otherwise specified, molar extinction coefficient (ε) values are typically measured in ddH …

WebCalculate extinction coefﬁcient of an oligo by either summing up the extinction coefﬁcients of the individual bases times their number of occurrences. Or use a formula …

james wallington obituaryWebµM Cy3-Cy5 in serum free DMEM for 1 h before imaging. (b) Same as (a) except cells were incubated with Cy3-Cy5 first, followed by treatment with MitoTracker®. (c) Same as (b) except a mixture of Cy3 and Cy5 was used instead of Cy3-Cy5. (d) Same as (b) except Cy3-Cy5-R8 was used instead of Cy3-Cy5. Excitation wavelength for MitoTracker® … lowes queen bed framesWebNov 13, 2009 · The extinction coefficient for Cy5, was determined and taken as 230.400 at 640 nm, while for Sulfo-Cy5 is 187.000 (FEW Wolfen) at 645 nm in water. For calculation of the label degree, only the … james wallis actorWebCy5 (625);650 670 792 QY 0.27: Cy5.5 675 694 1128 QY 0.28: Cy7 743 767 818 QY 0.28 ... Due to its high molar extinction coefficient, this dye is also easily detected by naked eye on electrophoresis gels, and in solution. Cy5 became a popular replacement for far red fluorescent dyes because of its high extinction coefficient ... james wallis financial planningBecause they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while … james wallington cvcWebƐ350 = molar extinction coefficient Cy5 dye = 150,000 M-1cm-1 A280 = conjugate absorbance at 280 nm A280c = corrected conjugate absorbance at 280 nm = A 280 - (A … lowes queens nyWebUnless otherwise specified, molar extinction coefficient (ε) values are typically measured in ddH 2 O or PBS. Cy5 (Cyanine-5) is spectrally similar to DiD, TOTO-3, Atto 647, Atto … james wallis houston