WebJan 15, 2024 · CircRNAs contain microRNA (miRNA) binding sites and can function as miRNA sponges to regulate gene expression by removing the inhibitory effect of an miRNA on its target gene. Methods:We used the bioinformatics software TargetScan and miRanda to predict circRNA-miRNA and miRNAi-Mrna interactions. WebTrying to unveil the underlying molecular mechanism, pull-down p53 immunoprecipitation evidenced that miR23-5p specifically and directly binds p53 in the cell nuclei of …
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WebJul 9, 2024 · RNA pull-down is an in vitro technique that uses labeled RNA as a bait to detect protein-RNA interactions. Currently, biotinylated probes based on reverse splicing … WebJun 22, 2024 · Expression levels of circRNAs and mRNAs were normalized to the expression levels of GAPDH, while small RNA RNU6 (U6) was used for miRNA. The detecting primers for circRNAs were designed based on its head-to-tail junction, and all primer sequences were displayed in Supplementary Material. Actinomycin D … truman\u0027s civil rights legislation
RNA Pulldown - Creative Biogene IntegrateRNA
WebJan 9, 2024 · Ectopic fat deposition in the liver, known as non-alcoholic fatty liver disease (NAFLD), affects up to 30% of the worldwide population. miRNA-122, the most abundant liver-specific miRNA, protects hepatic steatosis and inhibits cholesterol and fatty acid synthesis in NAFLD. WebCircRNA pull down and miRNA deep-sequencing demonstrated the binding of miR-92b-3p and circCDYL. Interestingly, circCDYL did not act as miR-92b-3p sponge, but was degraded in miR-92b-3p-dependent silencing manner. Clinically, expression of circCDYL and miR-92b-3p was associated with clinical outcome of HER2+ breast cancer patients. WebAug 14, 2024 · circRNA在乳腺癌发展过程中有巨大的调控潜能,在乳腺癌(BC)细胞增殖、迁移、侵袭等方面发挥重要作用。 然而,在BC中,circRNAs与自噬的关系以及自噬相关的circRNAs在乳腺癌临床诊断和治疗中的作用仍然需要探索。 展开全文 声明:该文观点仅代表作者本人,搜狐号系信息发布平台,搜狐仅提供信息存储空间服务。 首赞 阅读 () 我来 … philippine business registry philhealth