Cigarette smoke extract experiments
WebThe experiment was conducted using an in situ Cell Death Detection kit (11684817910, Roche, ... Chen H, Liao K, Cui-Zhao L, et al. Cigarette smoke extract induces apoptosis of rat alveolar type II cells via the PLTP/TGF-beta1/Smad2 pathway. Int … WebMale Balb/c mice were divided in 4 groups: control, cigarette smoke (CS), AAILE, and AAILE + CS. Animals in the CS group were exposed to cigarette smoke for a period of 10 weeks. AAILE oral administration at a dosage of 100 mg/kg body weight was started 2 weeks prior to CS exposure and continued until the termination of the experiment.
Cigarette smoke extract experiments
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WebCigarette smoke extract (CSE) is an aqueous solution containing most of the compounds and toxins inhaled by smokers, and commonly used as a preferred alternative for cigarette smoke (CS) 2. CSE has been used in cell experiments in vitro, and has been proven to have a direct effect on cell dysfunction 3.
WebMay 5, 2024 · Cigarette smoke (CS) is a complex chemical mixture, containing over 4000 identified compounds. The most prominent of these are nicotine, acrolein, N … WebMar 31, 2024 · Cigarette smoke extract (CSE) was prepared by bubbling smoke from two cigarettes (1.2 mg nicotine and 14 mg tar) without a filter into 20 mL of serum-free Ham’s F-12 K medium at a rate of 1 cigarette/min as previously described [ 11 ]. The pH of the medium was adjusted to 7.4 using HCl, and the medium was sterile filtered with a 0.22 …
WebUnder a cigarette smoke extract (CSE) stimulation condition, the apoptosis was analyzed by flow cytometry assay. Caspase-3 activity was examined with a caspase-3 activity assay kit; besides, inflammation and oxidative stress were assessed by measuring interleukin-6, tumor necrosis factor-α, glutathione/oxidized glutathione, reactive oxygen ... WebKeyword:'cigarette smoke extract' Showing 1-1 of 1 result for "cigarette smoke extract" within Products. Products Genes Papers Technical Documents Site Content …
WebNov 29, 2024 · Cigarette smoking is a risk factor for vascular diseases, including hypertension, atherosclerosis, and aneurysm [ 1, 2 ]. A crucial feature of smoking …
WebSep 12, 2011 · Cigarette smoke extract (CSE) has been used as a model for the effects of smoking in lung diseases and shown to increase production of pro ... cells were stimulated for 4 hours, harvested and stored at −80°C prior to RNA extraction. Experiments were also carried out for 24 hours and supernatants were stored at −80°C. For some experiments ... greed ultimate shieldWebOct 3, 2016 · Cigarette Smoke Extract: A Preclinical Model of Tobacco Dependence. Animal models are used to study many human diseases, one of which is tobacco … flo-tech image pressure relief cushionWebCigarette smoke extract (CSE) triggered the upregulation of CatS by THP-1 macrophages through the mTOR/TFEB signaling pathway. In a co-culture model, following the exposure of macrophages to CSE, an enhanced level of permeability of lung epithelial (16HBE and NHBE) cells towards FITC-Dextran was observed, which was associated with a … flo tech liteWebJan 1, 2024 · Experiments were repeated using cigarette smoke extract (CSE) for comparison. IL-8 was measured by ELISA, activation of NF-kappaB by ELISA and Western blotting, and caspase-3 activity by Western ... gree ductedWebIn this study, we demonstrate how a mixture of plant sterols (MPtS) consisting of β-sitosterol, campesterol and stigmasterol, at serum concentration reached after ingestion of a drink enriched with plant sterols, inhibits eryptosis induced by cigarette smoke extract (CSE). Isolated RBCs were exposed for 4 h to CSE (10–20% v/v). flo-tech liteWebTo Study the Toxic Effects of Cigarette Smoke - Week 2 During this laboratory period you will conduct the experiment you designed last week. Organize the members of your … greed unleashedWebAbstract. Smoking tobacco is a major risk factor for the development of lung cancer, COPD, and other lung pathologies in smokers. Cigarette smoke (CS), which is … gree ductless warranty